TG-A mice have decreased LT-HSC activity. Quantification of BM LSK cells per hind limb (1 femur and 1 tibia). Cells were obtained by both flushing and crushing, the same method used for TG and WT in the same cohort (A). (B-D) LSK cell frequency (B), BM pST-HSCs (C), and pLT-HSCs (D) in TG-A mice and their WT littermates from group A. (E) Donor BM from TG-A mice and their WT littermates was collected by flushing, mixed with competitor BM, and transplanted into 10 recipients per group. The percentage of CD45.2⁺/CD45.1⁻-expressing myeloid cells (CD11b⁺), T cells (CD3ϵ⁺), and B cells (B220⁺) in the blood of recipient mice was analyzed at 4, 8, 12, and 16 weeks after transplantation as indicated. Each dot represents an individual recipient mouse. Bar indicates the mean and SEM. *P ≤ .05.