TG-B mice have decreased BM LSK cells and increased splenic LSK cells at 4-5 weeks of age. Total BMMCs per limb (A), LSK cells per limb (B), and BM LSK-cell frequency (C) were quantified from 4- to 5-week-old TG-B and WT male and female littermate mice (n = 11 TG-B mice and sex-matched littermate controls; each dot indicates an individual donor; 2 separate experiments are indicated with 2 colors). (D) BMMCs flushed from hind limbs of WT or TG mice, as indicated, were mixed in a 2:1 ratio with CD45.1⁺ competitor BM cells and transplanted into CD45.1⁺ recipients. The percentage of CD45.2⁺/CD45.1⁻-expressing myeloid cells (CD11b⁺), T cells (CD3ϵ⁺), and B cells (B220⁺) in the peripheral blood of recipient mice was analyzed at the times indicated after transplantation. Each dot represents an individual recipient mouse. Bars indicates the mean and SEM. *P ≤ .05; **P ≤ .01; ***P ≤ .001.