Progression of bone phenotype in TG mice results in neutropenia and dramatically increased LSK cells, pST-HSCs, and pLT-HSCs in spleens. (A-B) H&E staining of longitudinal sections of representative distal femora (A) and lumbar vertebrae (B) from 8-week-old WT and TG littermate mice. Bars indicate 0.2 mm (low power) and 0.1 mm (high power). Quantification of spleen cellularity (C), frequency of spleen LSK cells (D), pST-HSCs (E), and pLT-HSCs (F) from 8-week-old WT and TG littermate mice. Quantification of blood WBC (G), monocyte (H), granulocyte (I), and lymphocyte (J) counts in male and female WT and TG littermate mice. Each dot represents an individual mouse. Bar indicates the mean and SEM. *P ≤ .05; **P ≤ .01; ***P ≤ .001.