Figure 4
AKT-dependent γc cytokine and TCR signals induce NKp30 expression in Vδ1+ T cells. (A-B) Flow cytometry analysis of NKp30 expression on pregated Vδ1+ T cells from γδ PBL cultures after 7 days in the presence of IL-2 or IL-15, alone or in combination with PHA or OKT3 (anti-CD3ϵ mAb). (C) Effect of blocking anti-TCRγδ mAb on NKp30 induction in PHA and IL-2–activated γδ PBLs. The shaded gray area is pregated NKp30+ cells in 7-day control cultures. (D) Effect of chemical inhibitors LY294002 and UO126 on NKp30 induction in PHA and IL-2–activated γδ PBLs, prelabeled with CFSE. Data in this figure are representative of 2 to 3 independent experiments with similar results.
