Deletion of HIF-1α partially restores impaired HSC quiescence caused by Cited2 deficiency. (A) Percentage of LT-HSCs (CD48⁻CD150⁺ LSKs) in LSK cells determined by FACS analysis. (B) Percentage of annexin V⁺DAPI⁻ cells within LSK cells and CD34⁻ LSK cells. (C) Relative mRNA level of Puma in LSK cells. (D) Percentage of LSK cells in G₀. (E) Percentage of CD34⁻ LSK cells in G₀. (F) The percentage of BrdU⁺ LSK cells. All data are shown as means ± SEM (n = 4). WT indicates wild-type.