Deletion of HIF-1α partially rescues impaired HSC reconstitution capacity caused by Cited2 deficiency. (A-B) 5-FU treatment. (A) WBC counts at the indicated times after 5-FU administration (n = 10). (B) Survival of mice after 5-FU administration (n = 10). The survival of double-knockout mice was significantly improved compared with Cited2-knockout mice (P < .01). (C-E) Transplantation experiments. (C) Absolute numbers of donor-derived B cells (B220⁺), myeloid cells (Mac-1⁺ or Gr-1⁺), and T cells (CD3⁺) in PB 16 weeks after noncompetitive transplantation (means ± SEM, n = 5). (D) PB chimerism in recipients 8, 12, and 16 weeks after competitive transplantation (n = 6). (E) BM chimerism in recipients 16 weeks after competitive transplantation. Data are shown as means ± SEM (n = 6). WT indicates wild-type.