The induction of autophagy is observed during monocyte differentiation in vivo. (A) Representative immunoblot analysis of lysates from mouse BM monocytes incubated with or without murine M-CSF at a concentration of 20nM for 14 hours. Where indicated, cells were treated in the presence or absence of 3-MA (10mM) or CQ (50μM). (B) Representative flow analysis of cells from thioglycolate-induced peritonitis costained with F4/80-APC and Ly6C-FITC for the indicated times. (C) Ly6C^highF4/80^low BM monocytes and the F4/80⁺ population from panel B were sorted and their lysates compared by Western blot analysis. Representative immunoblot analyses are shown.