Multilineage differentiation capacity of Lin⁻PDGFRαβ⁺ BMSCs. (Ai-iv) Histology of subcutaneous transplants of either empty scaffolds (Ai) or Lin⁻PDGFRαβ⁺ MSC (Aii-iv). Gelfoam scaffolds loaded with Lin⁻PDGFRαβ⁺ MSCs were either decalcified and embedded in paraffin for hematoxylin and eosin staining (Aii-iii) or nondecalcified and embedded in methylmethacrylate resin for Von Kossa staining (Aiv). (B) Three-dimensional pellet cultures of Lin⁻PDGFRαβ⁺ MSCs embedded in paraffin and stained with Toluidine blue (0.1% weight/volume; Bi), Alcian blue (Bii), collagen type II (Biv), and mouse IgG1 isotpe (Biii). (C) Oil red O staining of Lin⁻PDGFRαβ⁺ MSCs after adipogenic differentiation for 14 days.