Prospective isolation of Lin⁻PDGFRαβ⁺ clonogenic progenitors from DBM. (A) Gating strategy (left panel) and FACS analysis of whole BM from C57Bl/6 and BALB/c mice demonstrating the frequency of Lin⁻PDGFRαβ⁺ BMSCs obtained from either standard flushing or sequential enzymatic disaggregation of BM plugs. (B) Gating strategy (left panel) for prospective isolation of Lin⁻PDGFRαβ⁺ population from C57Bl/6 and BALB/c inbred mouse strains. (C-D) Incidence of CFU-F from prospective isolation of Lin⁻PDGFRαβ⁺ BMSCs from C57Bl/6 and BALB/c mice. Colonies > 50 stromal cells; clusters represent 10 to 49 stromal cells.