Specific deletion of WASP and N-WASP in B cells results in a decreased number of peripheral B cells. (A) Total absolute number of spleen B cells in WT, WKO, cNWKO, and cDKO mice. (B) Schematic diagram depicting B-cell development in the spleen (left) and flow cytometric analysis to define T1, FO, T2-MZP, and MZ B cells (right) in absolute number (C) and relative number (D) of cells. (E) Flow cytometry of peritoneum cells to define B1a, B1b, and B2 cells in absolute (F) and relative (G) numbers. Each group represents averages ± SD from 14 (WT, WKO, and cDKO) and 10 (cNWKO) mice for panels A through D and 6 mice per group in panels E through G. *P < .05 compared with WT.