Global gene-expression analysis of hemangiogenic versus cardiogenic Flk-1⁺ mesoderm. (A) Flk-1⁺ cells were sorted from day 3 Er71^+/+, Er71^−/−, and iEr71 EBs (± DOX, DOX was added from days 2-3) and subjected to microarray analysis. Representative clusters of the indicated lineage-specific genes are shown in the heat map. Red shading indicates increased expression and blue shading indicates decreased expression. (B-E) Expression of the indicated genes was validated by qRT-PCR in Flk-1⁺ mesoderm prepared as in panel A.