Coexpression of β-catenin in the context of ER71 overexpression rescues cardiogenesis. (A-D) iEr71 and iβ-catenin-2A-Er71 ES cells were differentiated with (+DOX) or without (−DOX) DOX from days 2-3 of differentiation and analyzed for Flk-1 (A) and PDGFRα on day 3.25 (note that there is a great increase in cells expressing Flk-1 and PDGFRα from day 3-3.25 compared with Figure 1I), CD41 and CD45 on day 6 (B), VE-cadherin and CD31 on day 6 (C), and cTnT and SMAα on day 8 (D). Data are presented as means ± SD of at least 3 independent experiments.