The granular secretion is normal in Jam-A^gt/gt platelets. (A) ATP secretion from Jam-A^wt/wt and Jam-A^gt/gt platelets stimulated by various concentrations of AYPGKF. Quantitation of experiments performed 3 times independently. (B) Flow cytometric histogram of P-selectin exposure on Jam-A^wt/wt and Jam-A^gt/gt platelets stimulated with 100μM AYPGKF for 10 minutes. Quantitation of mean fluorescence intensity normalized over control IgG from at least 3 independent experiments. (C) Quantitation of thrombin (0.1 U/mL)–induced TxA2 generation in Jam-A^wt/wt and Jam-A^gt/gt platelets (n = 3). (D) Flow cytometric histogram of PECAM-1 expression on Jam-A^wt/wt and Jam-A^gt/gt platelets. Quantitation of mean fluorescence intensity was normalized over control IgG from at least 3 independent experiments.