Absence of Jam-A promotes platelet spreading on immobilized Fg. (A) Representative DIC images of Jam-A^wt/wt and Jam-A^gt/gt platelets spread on immobilized Fg. BSA was used as a control. Zoomed views are indicated with arrowheads. (B) Quantification of the percentage of fully spread platelet on immobilized Fg compared with BSA. At least 100 individual platelets per view in triplicate for each time point were analyzed. Data shown are the quantification from at least 3 independent experiments. (C) Platelet surface area from panel A. Quantitation of > 50 platelets in a given view in triplicate were analyzed from at least 3 independent experiments.