Effect of Tmprss6 deletion on spleen size, serum EPO levels, and iron parameters of thalassemic mice. In the figure are graphed: spleen weights normalized to body weight (A); serum EPO levels (B); splenic (C); and hepatic (D) non-heme iron content (SIC and LIC); liver mRNA expression of hepcidin (Hamp; E), Hamp normalized on LIC (Hamp/LIC; F), Bmp6 (G), and inhibitor of DNA binding 1 (Id1; H). mRNA expression ratio was normalized to a male WT mean value of 1. Mean values of 4 to 8 animals for sex and genotype (WT, Tmprss6^−/−, Hbb^th3/+ [th3/+], and Tmprss6^−/−Hbb^th3/+ [Tmprss6^−/−th3/+]) are graphed and error bars indicate SD. Asterisks refer to a statistically significant difference. (*P < .05; **P < .01; ***P < .005). For complete statistical analysis see supplemental Table 2.