Comparison of the number of CD4⁺CD25⁺CD127^loFoxP3⁺ T cells per liter after transplantation in RICAZA patients compared with time-matched samples from RIC controls. (A) The number of Tregs per liter was quantified at sequential intervals after transplantation and plotted in a histogram that displayed the mean and the SEM. In total, 16 RIC controls and 19 RICAZA patients were analyzed at similar time points after transplantation. For the RICAZA patients, samples were collected at cycle 1 before treatment. A significant increase in the number per liter of CD4⁺CD25⁺CD127^loFoxP3⁺ Tregs at cycle 3 was observed in AZA-treated patients (n = 17) compared with the control population (n = 11; P = .0127, Mann-Whitney t test). Dark gray columns indicate control patients; and light gray columns, RICAZA patients.