CDK6, TAB2, and CCNT2 expression levels in monocytic and granulocytic induction cultures of CD34⁺ cells. (A-B) Western blot analysis of CDK6, TAB2, and CCNT2 in extracts from monocytic (A) and granulocytic (B) induction cultures at the indicated times. Whole cell extracts were incubated with anti-CDK6, TAB2, or CCNT2 antibodies. (C) CCNT2 protein expression was detected by Western blot analysis at day 4, 9, and 11 of monocytic induction cultures of CD34⁺ cells infected with Lenti-29a, Lenti-142, or Lenti-control. (D) CDK6 protein expression was detected by Western blot analysis at day 4, 9, and 11 of monocytic and granulocytic induction cultures of CD34⁺ cells infected with Lenti-29a or Lenti-control. (E) TAB2 protein expression was detected by Western blot analysis at day 4, 9, and 11 of monocytic and granulocytic induction cultures of CD34⁺ cells infected with Lenti-142 or Lenti-control. For the Western blots shown in this figure, GAPDH antibody was used to assess equal protein loading. The signal in each lane was quantified using Gelpro software, and the ratio of CCNT2, CDK6, and TAB2 to GAPDH was determined. Because the CD34⁺ cells are a mixture containing various HPCs and the sample amount was limited, the 0-day samples was not included in the Western blot analysis.