miR-155 expression is up-regulated in effectors T cells of mice recipients with aGVHD. (A) Schematic presentation showing aGVHD murine model used. (B) Histopathologic evaluation of a representative liver sample collected from a mouse with clinical score of GVHD of more than or equal to 7. This section was stained with hematoxylin and eosin (original magnification ×40 and ×400). (C) Average miR-155 expression in CD4⁺ CD62L⁻ T cells isolated from the spleen of 3 recipient mice with aGVHD (BM + spleen) or controls (BM alone). The results shown represent the average of 3 independent mouse samples performed each in triplicate. Values are expressed as relative miRNA expression after 2ΔCt calculations and normalization with sno135. Bars represent SD. (D) Hierarchical clustering analysis of miRNA expression obtained using Nanostring from CD4⁺ CD62L⁻ T cells isolated from the spleen of recipient mice with aGVHD (BM + spleen) or controls (BM alone). Color areas indicate relative expression of each miRNA among the 2 type of samples (red high, blue low expression). (E) Histopathologic and molecular evaluation of a representative liver sample collected from a mouse with clinical score of GVHD of more than or equal to 7 or a control mouse (BM alone). At least 3 mice in each group were evaluated for staining with LNA anti–miR-155 and scramble control. These sections were stained with LNA anti–miR-155 probes or scrambled controls. Dark staining indicates positivity (original magnification ×400). Note the strong staining of the mononuclear cells in the periportal lymphoid aggregate in the BM + spleen mouse that is lost in the same cells in the serial section with the scrambled probe.