Salidroside prevents oxidative stress–induced HSC loss. (A) salidroside partially limits H₂O₂-induced LSK expansion. BM cells from WT C57BL/6 mice pretreated with or without salidroside (75μg/g body weight) followed by H₂O₂ (0.25 μmol/g body weight) injection were harvested for LSK (Lin-Sca1⁺c-kit⁺) cell frequency assessment using flow cytometry. (B) Quantification of LSK frequency in mice described in panel A. (C) SD prevents H₂O₂-induced LSK CD34-Flt3- cell loss. BM cells described in panel A were subjected to clow cytometry analysis for LSK CD34⁻Flt3⁻ frequency. (D). Quantification of LSK CD34⁻Flt3⁻ frequency in mice described in panel A. (E) SD prevents H₂O₂-induced LSK CD150⁺CD48⁻ cell loss. BM cells described in panel A were subjected to Flow Cytometry analysis for LSK CD150⁺CD48⁻ frequency. (F) Quantification of CD150⁺CD48⁻ frequency in mice described in panel A. Results are means ± standard deviation (SD) of 3 independent experiments (n = 9 per group).