Salidroside prevents HSC cycling in vivo. (A) Salidroside partially reduces H₂O₂-induced LSK cell apoptosis. BM cells were harvested from WT C57BL/6 mice pretreated with or without salidroside (75μg/g body weight) followed by H₂O₂ (0.25 μmol/g body weight), and gated for LSK population. Apoptotic LSK cells were determined by annexin-V and 7AAD staining. (B) Salidroside and H₂O₂ have no effect on apoptosis of CD34-LSK cells. CD34 negative LSK cells were gated for apoptosis analysis. (C-D) Salidroside prevents HSC cycling in vivo. Cells described in panel B were subjected to Hochest and pyronin Y (C) or BrdU (D) staining. Representative images (top) and quantifications (bottom) were shown. Results are means ± SD of 3 independent experiments (n = 9 per group).