Incidence of the TBL1XR1/TP63 fusion in DLBCL. (A) FISH and Sanger sequencing of the fusion in 3 cases of DLBCL. Left hand column: results from a case known to be negative for the fusion on analysis of RNA-seq data. Middle column: results from a case where the fusion was detected in RNA-seq data. Right hand column: results of a patient who was not part of the RNA-seq cohort. Top panels: examples of representative cells from breakapart FISH assays on TMA. These images were produced at room temperature using an Olympus BX61 microscope with a UPlanFL N 40×/0.75 objective. The fluorochromes are Spectrum green and Spectrum orange. The images were acquired using a CV-M4+CL camera (JAI) and ARIOL software (Version 3.4; Genetix). Bottom panel: electropherograms from Sanger sequencing of an amplicon that spans the junction between TBL1XR1 and TP63 in the gene fusion transcript. (B) Incidence of the TBL1XR1/TP63 fusion in the total cohort and the cohorts where the 2 detection techniques for the fusion were applied. The nonadditive nature of the cohorts toward the total is the result of an overlap of 30 DLBCLs between the 2 cohorts, including 2 DLBCLs that harbor the fusion. Cell of origin designations are as follows: total GCB, 56 by gene expression profiling and 59 by the tally algorithm; total non-GCB, 38 ABC and 14 unclassifiable by gene expression profiling and 86 non-GCB by the tally algorithm; RNA-seq, GCB, ABC, and unclassifiable are all by gene expression profiling; TMA GCB, 23 by gene expression profiling and 59 by the tally algorithm; and TMA non-GCB, 12 ABC and 7 unclassifiable by gene expression and 86 non-GCB by the tally algorithm.