Neutrophil slow rolling and arrest inex vivoflow chambers. (A) Time-lapse images of GFP⁺ wild-type and GFP⁻Tln1^fl/flMx1-Cre (top) or Fermt3^−/− (bottom) neutrophils rolling side-by-side in an autoperfused flow chamber coated with E-selectin and ICAM-1. Scale bar represents 20 μm. (B) Mean rolling velocities of neutrophils in flow chambers with immobilized E-selectin or E-selectin and ICAM-1. A wall shear stress of 6 dyne/cm² was used in all experiments. Data are mean ± SEM of at least 25 cells per condition (n = 3 mice each). (C) Neutrophil arrest in flow chambers with immobilized E-selectin, ICAM-1, and CXCL1 after 5 minutes of perfusion. Data are mean ± SEM (number of adherent cells in each of at least 3 fields of view per flow chamber; n = 3 mice each).