CD86− HSCs effectively engrafted the CD150+ CD48− LSK compartment in transplant recipients but produced fewer blood cells and especially B lymphocytes than the CD86+ cohort. (A) Subsets of CD150+ CD48− LSK stem cells were resolved and sorted according to CD86 expression. Twenty of each subset were transplanted separately to lethally irradiated recipients along with 2 × 105 whole BM rescue cells. Chimerism with respect to phenotypically defined HSCs was found 6 months later. Not shown are data points for 3 mice in each group in which chimerism was < 0.01%. Peripheral blood samples were tested at monthly intervals, and percentages of donor type cells are shown for CD86+ (open circles) and CD86− (closed squares) HSC transplant recipients. Statistically significant (*P = < .05) differences were found from the third month. (B) The bars show percentages of the donor type cells that expressed myeloid, (GR-1 and/or CD11b), B (B220 plus CD19) or T (CD3) lineage markers in the blood of individual recipients. Note that myeloid cells predominated in 6 of 8 recipients of CD86− HSCs, whereas CD86+ HSCs were balanced or lymphoid skewed.
