CD86 expression distinguishes HSCs with full differentiation potential among those with high CD150. (A) Sorting threshold of CD150⁺ and CD150^Hi CD48⁻ LSK cells are shown as right and left panels, respectively. CD86⁻ and CD86⁺ CD150^Hi HSCs were sorted from 10-month-old mice. (B) Schematic representation of transfer experiments. Two hundred CD86⁻ and CD86⁺ CD150^Hi HSCs (CD45.2) were transplanted separately into CD45.1/CD45.2 F₁ mice along with 2 × 10⁵ whole BM cells. (C) Five months later, peripheral blood samples were stained with anti-CD11b, Gr-1, B220, CD19, CD3e, NK1.1, CD45.1, CD45.2, and propidium iodide. Lymphoid cells predominated in recipients of CD86⁺ HSCs compared with CD86⁻ HSCs (P < .001). (D) Absolute numbers of lymphocytes in peripheral blood (PBL) of recipients of CD86⁻ HSCs (closed symbols) and CD86⁺ (open symbols) were indicated as green (B cells), red (T cells), and blue (myeloid cells). (E) Chimerism of donor cells in peripheral blood (PB) and BM are indicated with circles and triangles. Donor cells from CD86⁻ and CD86⁺ cells are indicated as closed and open symbols, respectively.