CD86 is useful for discriminating stem/progenitor cells regardless of mouse strain and developmental age. (A) LSK as well as lymphoid and myeloid progenitors in E14.5 fetal liver of C57BL6 mice were identified by flow cytometry. The histograms show selective expression of CD86. (B) Lin⁻, CD150⁺, CD48⁻, c-Kit⁺ HSCs in BM of Balb/c mice were gated as shown (left panels). Single parameter histograms to the right show CD86 expression. Similar results for lymphoid progenitors defined as Lin⁻, Flt3⁺, IL-7Rα⁺, c-Kit⁺ are shown in the far right panel, along with whole BM (WBM).