Figure 3
![Figure 3. Depletion of HSCs in the BM of RbΔ/ΔE2f8Δ/Δ mice. (A-B) The absolute numbers of phenotypic stem cells (Lin−c-kit+Sca-1+ [LKS+]), progenitors (Lin−c-kit+Sca-1− [LKS−]), and long-term stem cells (Flk2−Lin−c-kit+Sca-1+) in the BM (A) and spleens (B) were estimated by flow cytometric analysis. Values above each bar in panel B represent the fold increases compared with control mice. (C-F) Photographs (C-D) and numbers (E-F) of CFU-S colonies from mice with the indicated genotypes. (G-H) Numbers of CFU-GEMM, CFU-GM, and BFU-E in the spleens (G) and BM (H) of mice with the indicated genotypes.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/119/19/10.1182_blood-2011-10-388231/4/zh89991290160003.jpeg?Expires=1735535524&Signature=Ykhj96GCMMzmKrf7-BKpZBcinGhvgwOUNqRQPfX56F3HlW2WK~H8gE8IVqFvumKJFWMDtrjvSW3UAiZG7FQ0iIm9XeGuUQpEYFOBmQ673cLLZGV8VaV2EP~FIcIOBJy-4mdjF7DGTOQV78RSS~yDKGtTxn3~rUSpOqY4zTSUjO2rbwaXuGwAUF1oMFgNlkV1fkGJdeFCcgrUjvT1lIMYdlDUx8qgTSTRsKiYtD0k0soXiss0gDvcUzIggTSQQrU8EfxJBXHe330ITxHcpJpvz0qXyxJa6Hz1F2z8aRClq0-9YRQ7PLt0zia797IHPWo27tLmRO3jb1Zwp1ROL17Jng__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Depletion of HSCs in the BM of RbΔ/ΔE2f8Δ/Δ mice. (A-B) The absolute numbers of phenotypic stem cells (Lin−c-kit+Sca-1+ [LKS+]), progenitors (Lin−c-kit+Sca-1− [LKS−]), and long-term stem cells (Flk2−Lin−c-kit+Sca-1+) in the BM (A) and spleens (B) were estimated by flow cytometric analysis. Values above each bar in panel B represent the fold increases compared with control mice. (C-F) Photographs (C-D) and numbers (E-F) of CFU-S colonies from mice with the indicated genotypes. (G-H) Numbers of CFU-GEMM, CFU-GM, and BFU-E in the spleens (G) and BM (H) of mice with the indicated genotypes.
