Reexpression of p15Ink4b results in increased differentiation and maturation of BM-DCs. (A) Lineage-negative cells from p15Ink4b^fl/fl-LysMcre mice were infected with Mig0- or MigDDp15-expressing viruses, FACS sorted for GFP⁺ cells, and cultured in the presence of GM-CSF (20 ng/mL) and IL-4 (10 ng/mL) for 5 days to generate BM-DCs, which were then FACS analyzed for expression of CD11c and CD11b. Numbers indicate the percentage of double-positive cells ± SD from 2 independent experiments. (B) Bright-field images of the cells on day 5 of culture. Scale bars represent 10 μm. (C) BM-DCs were FACS analyzed after treatment with LPS (100 ng/mL for 16 hours) for expression of MHC II, CD80, CD86, and CD40 molecules. (D) Bar graphs depicting relative mean fluorescence intensity values of MHC II, CD80, CD86, and CD40 expression on BM-DCs derived from p15Ink4b^fl/fl-LysMcre progenitors infected with Mig0 MigDDp15. Data are shown as means ± SD of 2 independent experiments. *P < .05.