Th17- and Th1-like Treg cells coproduce pro- and anti-inflammatory cytokines. (A) Representative flow cytometric analysis of IL-10, IL-17, and IFN-γ production by sorted Th (left) and Treg (right) cell subsets stimulated for 5 hours with PMA/ionomycin. (B) Frequency of IL-17–, IL-10–, and IFN-γ–producing cells among gated FOXP3⁺ Treg cells in each of the indicated Treg cell subsets. Each symbol represents 1 donor; horizontal bars indicate the mean. Data are from 10 donors. *P < .05; ***P < .001 (ANOVA). (C) Frequency of IL-10–producing cells among IL-17⁺ cells in Th17 cells or Th17-like Treg cells (left) or among IFN-γ⁺ cells in Th1 cells or Th1-like Treg cells (right). Data are from 10 donors. ***P < .001 (2-tailed paired t test).