Th17- and Th1-like Treg cells express the lineage-specifying transcription factors RORγt and T-bet. (A) Quantitative RT-PCR analysis of RORγt (RORC), T-bet (TBX21), and GATA-3 (GATA3) expression by the indicated Th and Treg cell subsets. AU indicates arbitrary units. Data are means ± SEM of 7 donors. (B) Flow cytometric analysis of IFN-γ, IL-17, T-bet, and RORγt expression by sorted naive T cells (T naive), Th17-like, and Th1-like Treg cells stimulated for 5 hours with PMA/ionomycin. Data are representative of 3 independent experiments.