Characteristics of DC-d-Ms. Purified pre-cDCs were cocultured on irradiated stroma and recovered 10 to 12 days later for analysis. In some wells, TNF was added for the final 24 hours of culture. Splenic cDCs or pre-cDCs–derived cDCs were used as controls. (A) Giemsa-staining of cytospin preparations (original magnification 40×). (B) Expression of indicated antigens (white histogram) and isotype control (gray histogram). (C) Morphology of sorted DC-d-Ms and cDCs after overnight incubation in GM-CSF (original magnification 40×). (D) Phagocytosis of OVA conjugated with FITC (gray histogram). MFI indicates mean fluorescence intensity. (E) Expression of indicated surface antigens after 16-hour exposure to LPS (white histogram) or control (gray histogram). Vertical black lines indicates staining with isotype controls. (F) Stimulation capacity in mixed allogeneic lymphocyte reactions. Results are expressed as mean cpm × 103 ± SD. (G) Expression of CD284 (white histogram) and isotype control (gray histogram). Data are representative of > 3 independent experiments.
