Detection of cDCs-derived macrophages in CD11c-Cre Rosa26-EGFP mice. (A) Expression of CD11c, MHC class II, and CD11b by Lin⁻CD45⁺ EGFP⁺ cells in LLC tumors and spleens from CD11c-Cre⁺ Rosa26-EGFP mice and their CD11c-Cre⁻ littermate controls bearing LLC tumors (0.3-0.5 cm diameter). Vertical black line in far right dot-plots indicates staining with isotype control for CD11c. (B) Expression of CD11c and MHC class II on pre-cDC–derived CD45.2 EGFP⁺ cells. BM pre-cDCs (5-10 × 10⁵) from CD45.2 CD11c-Cre⁺ Rosa26-EGFP mice were injected intravenously into nonirradiated CD45.1 congenic B6 mice bearing subcutaneous LLC tumors transduced with a retrovirus encoding CCL3. At 3 days, spleens and tumors were recovered for analysis. (C) Percentage of Lin⁻CD45⁺ EGFP⁺ cells that do not express CD11c (mean ± SD; *P = .03). Data are representative of 3 independent experiments with 3 mice per experiment.