Quantitative real-time PCR analysis indicates higher expression of CIS-associated candidate genes in mice with insertions near those genes. Candidate gene transcript expression in spleens or lymph nodes of mice with insertion events near or away from those genes. Intron-spanning primers were designed. Expression was calculated using the ΔΔCT method⁴¹  and is shown in log scale. Expression level was normalized to the respective WT tissue. Each dot represents one PCR. Reactions were performed on cDNA isolated from leukemia with insertions near other CIS genes labeled with “NO.” (A) Reactions performed on cDNA isolated from leukemia with insertions near Mn1 and the novel gene C130026L21Rik labeled with “YES” (*significant P value < .05). (B) Reactions performed on cDNA isolated from leukemia with insertions near Fosb labeled with YES (P = .153). (C) Reactions performed on cDNA isolated from leukemia with insertions near Bcl11a labeled with YES (P = .172).