Effects of Mig and anti-Mig antibody after 5-FU treatment. Mice received a single dose 5-FU (300 mg/kg) on day 0 and then were treated for 3 days intravenously with either Mig (15 μg/kg; n = 16) or PBS as control (n = 15; A top). Mice treated with Mig showed lower survival rates than the control mice (P = .048; A bottom). When mice had been treated with either control (nonsensitized rat serum; n = 7) or anti-Mig rat serum (n = 11), the anti-Mig group showed significantly higher survival rates than mice in the control group (P = .005; B). (C-D) Anti-Mig serum significantly accelerated the recovery of BM (C) and increased colony counts per femur (D) compared with controls. Mice were injected with 225 mg/kg 5-FU on day 0 and treated with either control serum or anti-Mig serum. BM mononuclear cells were counted and cultured for GM-CFU on days 8, 9, 10, 12, and 14. Each point represents the mean + SEM in 2 independent experiments (n = 6; *P < .05).