Cebpa-Cre–driven deletion of Dicer1 does not affect the fraction of myeloid-committed HSPCs in mice. (A) Percentage of LSK cells (Lin⁻Sca1⁺C-Kit⁺), progenitors (Lin⁻Sca1⁻C-Kit⁺), and EYFP⁺ progenitor cells of Dicer1^wt (n = 3), Dicer1^wt/fl (n = 4), and Dicer1^fl/fl cells (n = 4) in E13.5 fetal livers. (B) EYFP⁺ cells from the BM of transplanted mice were sorted by flow cytometry. DNA was isolated and analyzed by PCR. DNA fragments from Cebpa-Cre;Dicer1^wt/fl;R26-LSL-Eyfp and Cebpa-Cre;Dicer1^fl/fl;R26-LSL-Eyfp are indicated by fl/wt and fl/fl, respectively. Tail DNA of Dicer1^wt/fl was used as a positive PCR control (c) for the floxed and wild-type alleles. Recombined lox-p sites are indicated by Δ. (C) Top panel: Percentage of LSK cells (Lin⁻Sca1⁺C-Kit⁺), and CMPs, GMPs, and MEPs in the progenitor fraction (Lin⁻Sca1⁻C-Kit⁺) of Dicer1^wt BM cells (n = 5), Dicer1^wt/fl (n = 6), and Dicer1^fl/fl cells (n = 7). Bottom panel: Percentage of EYFP⁺ cells in the indicated fractions.