Gata-1 and p53 are direct targets of miR-92a. Gata-1 and p53 (A) protein and (B) quantitative mRNA expression in empty vector control and miR-92a overexpressing CSC1 cells, where error bars represent SD; **P < .005. (C) Schematic representation (top panel) of the p53 3′UTR sequence containing potential miR-92a binding sites. Positions of mutations generated (p53-mu) are marked in gray. Luciferase assay (bottom panel) of the reporter vector containing the p53 binding site and its corresponding mutation (p53-mu) after transient transfection in NIH-3T3 cells stably expressing miR-92a. (D) Schematic representation (top panel) of the gata-1 CDS containing potential miR-92a binding sites (G₁ and G₂). Positions of mutations generated within G₁ (G1-mu) and G₂ (G2-mu) are marked in gray. Luciferase assay (bottom panel) of the reporter genes containing the G₁ or G₂ binding sites and their corresponding mutations (G1-mu or G2-mu) after transient transfection in NIH-3T3 cells stably expressing miR-92a. The expression is presented relative to that obtained with transfection of control vector alone. Error bars represent SD; **P < .005.