TIMP-3 modulated the DC phenotype. (A) The efficiency of TIMP-3 gene knockdown was confirmed by Western blot analysis. (B) rhTIMP-3 or TIMP-3 siRNA was applied during LPS-induced DC maturation. The expression of surface molecules was examined by flow cytometry. Histograms illustrate staining with isotype control (solid profiles) and with the indicated specific mAbs on untreated DCs (fine gray line) and treated DCs (bold black line). Results are representative of 3 separate donors.