Binding of FANCD2 to promoters is independent of BRG1. (A) Knockdown of BRG1. Normal lymphoblasts expressing a shRNA for Brg1 or a control shRNA were treated with or without 100μM H₂O₂ for 2 hours. Cell extracts were then subjected to Western blotting using Abs against BRG1 and actin. (B) Binding of FANCD2 to promoters is independent of BRG1. Cells described in panel A were treated with or without H₂O₂, followed by a ChIP assay using Abs against FANCD2. PCR was performed using primers specific for the promoter regions of GPX1, TXNRD1, GAPDH, or β-tubulin.