NF-κB transactivation by NEMO mutants from the XL-EDA-ID patients. NF-κB transactivation induced by NEMO mutants in the XL-EDA-ID patients. Mock vectors and wild-type (wt) NEMO were used as controls. The NF-κB activation index of NEMO variants were calculated as (NF-κB activation by each NEMO variant − NF-κB activation of the mock vector)/(NF-κB activation by wild-type NEMO − NF-κB activation of the mock vector). The data shown are the mean ± SD of triplicate wells and are representative of 3 independent experiments with similar results.