Pcgf1 binds to promoters of HoxA genes and promotes their ubiquitination. (A) ChIP was performed using Lin⁻ BM from Pcgf1-eGFP or control mice. DNA was pulled down with an Ab specific for eGFP, and the abundance of HoxA7, HoxA9, and HoxA10 promoter DNA was compared in these 2 samples by qRT-PCR. For HoxA7 and HoxA10, 2 different primer pairs were used. (B) Lin⁻ cells transduced with Pcgf1 (shPcgf1) or control shRNA (shCtrl) were subjected to ChIP using an Ab that recognizes ubiquitinated histone 2A (H2A K119ubi). Binding to Hox promoters was compared between samples by qRT-PCR with the indicated primers. Significance of changes was determined using the Student t test. *P < .05; **P < .01. n.d. indicates not detectable.