SHP-1 negatively regulates IL-6 mediated Th17 development in a T cell–intrinsic manner. (A) Graphic map of the construct used to generate DN-SHP-1 mice. (B) Representative IL-6 dose-response assay of DN-SHP-1–expressing and –nonexpressing T cells that were cultured under Th17-inducing, with the indicated IL-6 concentrations, followed by intracellular staining for IFN-γ and IL-17. Profiles show CD4⁺-gated population. (C) Relative Th17 T-cell generation from IL-6 dose-response assays as described in panel B (maximum % Th17 cells: 35% nonexpressers, 34% DN-SHP-1–expressers). EC₅₀: DN-SHP-1–expressers 1.0 ng/mL IL-6 (n = 3) and nonexpressers 2.5 ng/mL IL-6 (n = 4; P = .13). Data are representative of 2 independent experiments with multiple mice for each genotype.