Normal steady-state hematopoiesis in TN-C−/− mice. (A-C) FACS plots showing BM lineage frequency. (D) Quantification in each lineage (n = 4). (E) FACS plot showing the detection of LSK cells. (F-G) Total MNC count (F) and LSK cell count (G; n = 4). (H-K) FACS plots (H-I) and quantification (n = 4; J-K) of Flt3−CD34− LSK and CD48−CD41−CD150+ LSK cell frequency. (L-M) Cell-cycle status frequencies assessed through Ki-67 and Hoechst staining of LSK cells (n = 6). *P < .05.