TN-C^−/− mice show impaired hematopoietic recovery after 5-FU administration. (A-B) IHC for TN-C (red) and DAPI (blue) for day 7 BMs. TN-C^−/− mice lack TN-C immunoreactivity. (C) Survival curve for TN-C^+/+ and TN-C^−/− mice after 5-FU administration (n = 12; combination of 3 independent experiments). (D-F) Peripheral blood counts for WBCs (D), hemoglobin (Hb; E), and platelets (Plt; F) after 5-FU administration. (G) BM lineage frequencies on day 10 after 5-FU administration (n = 4). (H-I) IHC for c-Kit (green) and DAPI (blue) in TN-C^+/+ and TN-C^−/− BM on day 10 after 5-FU administration. (J-K) IHC for BrdU (red) and c-Kit (green) in TN-C^+/+ and TN-C^−/− BM on day 10 after 5-FU administration. (L) Quantification of the percentage of c-Kit⁺ cells among DAPI⁺ BM cells (n = 6). (M) Quantification of the percentage of BrdU⁺ cells among c-Kit⁺ cells (n = 6). (N-O) FACS plot and quantification showing the detection of LSK cells on day 10 after 5-FU administration (n = 4). (P) Survival curves for TN-C^+/+ and TN-C^−/− mice subjected to 6.5 Gy irradiation (n = 5; combination of 3 independent experiments). *P < .05. Scale bars indicate 100 μm in panels A, B, H, and I and 20 μm in panels J and K.