TN-C^−/− mice are vulnerable recipients for BMT. (A) Peripheral blood chimerism analyses of recipient mice infused with 2 × 10⁵ donor cells from TN-C^+/+ or TN-C^−/− mice (n = 10). (B-D) BMMNC count (B), LSK cell frequency (C), and LSK cell chimerism (D) of recipient mice 4 months after transplantation with 2 × 10⁵ donor cells from TN-C^+/+ and TN-C^−/− mice (n = 4). (E) Survival curves for TN-C^+/+ and TN-C^−/− recipient mice infused with 2 × 10⁵ cells (white and black boxes, respectively) or 1 × 10⁴ cells (white and black circles, respectively; n > 10 for each group; combination of 3 independent experiments). (F-G) Flow cytometric analysis of the BM in TN-C^+/+ or TN-C^−/− recipient mice 4 months after BMT (2 × 10⁵ cells) showing the frequency and chimerism of Flt3⁻CD34⁻ LSK cells and CD48⁻CD41⁻CD150⁺ LSK cells (n = 4). (H-I) BM lineage composition (H) and chimerism (I) of cells within each lineage in the BM of TN-C^+/+ or TN-C^−/− recipient mice 4 months after BMT (2 × 10⁵ cells; n = 4). (J) Survival curves for TN-C^+/+ and TN-C^−/− recipient mice challenged with 5-FU 4 months after BMT (2 × 10⁵ cells; n = 10; combination of 3 independent experiments). (K-M) Peripheral blood counts of mice shown in panel J (n = 10). *P < .05.