HLA A*0201-restricted epitope 341-351. Mapping the minimal immunogenic AA sequence and HLA stabilization. (A) The position of the different peptides in cyclin-A1. IFN-γ ICS of T-cell line (donor 2264) stimulated by autologous LCLs pulsed with the 10-mer 1, 10-mer 2, and 11-mer. Only pulsing with the 11-mer results in IFN-γ production in the T-cell line. (B) HLA stabilization assay: both 10-mer 1 and the 11-mer peptide stabilize HLA A*0201 on T2 cells. Negative controls are T2 cells pulsed with an irrelevant 15-mer (shaded).