T-cell clones recognize endogenous processed epitopes 227-235 and 341-351. (A) Expression of cyclin-A1 in several myeloid cell lines quantified by quantitative RT-PCR. (B) Clones 2196.D9 and D11_a (both specific for epitope 227-235) and clone 2264.E30 (specific for epitope 341-351) were tested for apoptosis induction in the THP-1 cell line using a caspase-3 assay. As negative controls, targets alone (shaded) and clone 2264.A1 specific for epitope 118-127 (HLA B*4001 restricted, THP-1 is B*4001-negative) were used. As a positive control, targets were incubated with 4μM camptothecin. The histograms correspond to the gates on the PKH26-prelabeled target cells.