Nuclear eIF4E regulates the export of lymphomagenic transcripts. (A) Protein levels of BCL6, BCL2, MYC, and eIF4E in the DH/TH DoHH2 cells transfected with siRNA for luciferase (as control) or siRNA for eIF4E. Protein quantification is shown on the right for triplicate experiments as mean ± standard error of the mean (SEM). (B) eI4FE mRNA immunoprecipitation for BCL6, MYC, BCL2, and GAPDH (as control) in DoHH2, SU-DHL6, and OCI-Ly1 nuclear fractions. Results expressed as fold enrichment over nuclear input. ***P < .001 and **P < .05 (Student t test for triplicates). (C) eI4FE RIP sequencing (RIP-seq) in OCI-Ly1 cells (vs nuclear input). Selected transcripts and pathways differentially enriched in eIF4E RIPs are shown on the right. (D) eIF4E nuclear targets that sustain BCL6, MYC, and BCL2 activity in lymphoma cells depicted as “networks” from Search Tool for the Retrieval of Interacting Genes/Proteins analysis. Transcripts identified in this eIF4E RIP-seq are shown in red circles. (E) Effect of ribavirin (RBV) on the mRNA nuclear export (determined by mRNA cytosolic/nuclear ratio) of BCL6, MYC, BCL2, and GAPDH (as control) in SU-DHL6, OCI-Ly1, and DoHH2 cells.