American Society of Hematology

Figure 6

$Figure 6. Ribavirin decreases TEHsp90 inhibition–induced Hsp70 upregulation. (A) Effect of ribavirin on the mRNA nuclear export (determined by mRNA cytosolic/nuclear ratio) of HSP70B (HSPA6) and GAPDH (as control) in OCI-Ly1, SU-DHL6, and DoHH2 cells. The effect on total mRNA is sown on the right with PU-H71 effect as control. (B) eI4FE mRNA immunoprecipitation for HSP70B and GAPDH (as control) in OCI-Ly1, SU-DHL6, and DoHH2 nuclear fractions. Results expressed as fold enrichment over nuclear input. (C) Polysomal profiling of HSP70B transcript in OCI-Ly1 cells treated with vehicle vs ribavirin (RBV). (D) Effect on Hsp70 and actin (as control) protein abundance in ribavirin- and vehicle-treated SU-DHL6, OCI-Ly1, and DoHH2 cells. Immunoblots from 50 μg of cell lysates were exposed for optimal time to visualize Hsp70 at baseline. Hsp70 protein quantification (to actin) is shown at the bottom for quadruplicate experiments as mean ± SEM.$

Ribavirin decreases TEHsp90 inhibition–induced Hsp70 upregulation. (A) Effect of ribavirin on the mRNA nuclear export (determined by mRNA cytosolic/nuclear ratio) of HSP70B (HSPA6) and GAPDH (as control) in OCI-Ly1, SU-DHL6, and DoHH2 cells. The effect on total mRNA is sown on the right with PU-H71 effect as control. (B) eI4FE mRNA immunoprecipitation for HSP70B and GAPDH (as control) in OCI-Ly1, SU-DHL6, and DoHH2 nuclear fractions. Results expressed as fold enrichment over nuclear input. (C) Polysomal profiling of HSP70B transcript in OCI-Ly1 cells treated with vehicle vs ribavirin (RBV). (D) Effect on Hsp70 and actin (as control) protein abundance in ribavirin- and vehicle-treated SU-DHL6, OCI-Ly1, and DoHH2 cells. Immunoblots from 50 μg of cell lysates were exposed for optimal time to visualize Hsp70 at baseline. Hsp70 protein quantification (to actin) is shown at the bottom for quadruplicate experiments as mean ± SEM.

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