Derivation and hematopoietic differentiation of patient-specific FA iPSCs. (A) Immunofluorescence of complemented patient-derived FA-A iPSCs. (B) Representative H&E stains of teratomas obtained by intramuscular injection of a complemented FA-A iPSC clone (4× and 20× magnification). (C) FANCD2 foci formation of normal donor (ND) or patient-specific complemented FA iPSC lines after exposure to vehicle or 2mM HU for 16 hours (60× magnification). (D) Number of CFUs per 1 × 105 normal donor and complemented patient-derived FA-A input cells (n = 3 independent clones, mean ± SD). (E) Photomicrographs depicting CFUs derived from patient-specific FA-A iPSCs and cytology of erythroid precursor cells, macrophages, and granulocytes (20× and 60× magnification). Images were obtained from FA-A iPSCs derived from patient sample number 2. GEMM indicates granulocyte/erythroid/macrophage/megakaryocyte; GM, granulocyte/macrophage; and BFU-E, burst-forming unit-erythroid.
