AGE levels are increased in plasma and within thrombi in diabetic mice. (A) Plasma concentrations of AGEs in diet and STZ-treated wt or cd36-null mice were measured by ELASA. Data are expressed as means ± SEM (n = 6-8 per group). (B) Carotid thrombi from wt or cd36-null mice induced as in Figure 3 were frozen, sectioned, and analyzed for the presence of AGEs by immunohistochemistry using anti-AGE IgG and a DAB⁺ substrate chromogen detection system. Brown color indicates immunoreactivity. Representative images are shown, and the red numbers indicate the intensity score of that image. (C) Mean AGE-staining scores (± SEM) of 5 sections from each thrombus (n = 5 per group).