FoxP3⁺ CD4⁺ and CD8⁺ T cells produce IFNγ but not IL-2 after restimulation in vitro. Irradiated CD45.2⁺ B6D2F1 mice (H-2^bxd) were injected with splenocytes and T cell–depleted BM from CD45.1⁺ FoxP3.GFP mice (H-2^b). On day 8 after transplantation, splenocytes were stimulated with PMA/ionomycin in the presence of BFA for 6 hours. The expression of IL-2 (A-B) and IFNγ (C-D) by CD45.1⁺ CD4⁺ (top panels) and CD8⁺ (bottom panels) FoxP3⁻ and FoxP3⁺ T cells were analyzed by flow cytometry. One representative contour plot of 3-4 independent experiments and compiled data of all experiments represented as mean ± SEM of n = 15-18 mice are shown.